Diaminopimelic Acid Amidation in Corynebacteriales

Identification of the amidotransferase AsnB1 as being responsible for meso-diaminopimelic acid amidation in Lactobacillus plantarum peptidoglycan.

The peptidoglycan (PG) of Lactobacillus plantarum contains amidated meso-diaminopimelic acid (mDAP). The functional role of this PG modification has never been characterized in any bacterial species, except for its impact on PG recognition by receptors of the innate immune system. In silico analysis of loci carrying PG biosynthesis genes in the L. plantarum genome revealed the colocalization of...

Hydrolysis of peptidoglycan is modulated by amidation of meso‐diaminopimelic acid and Mg2+ in B acillus subtilis

The ability of excess Mg2+ to compensate the absence of cell wall related genes in Bacillus subtilis has been known for a long time, but the mechanism has remained obscure. Here, we show that the rigidity of wild-type cells remains unaffected with excess Mg2+ , but the proportion of amidated meso-diaminopimelic (mDAP) acid in their peptidoglycan (PG) is significantly reduced. We identify the am...

Lactobacillus plantarum Peptidoglycan -Diaminopimelic Acid Amidation in meso AsnB1 as Being Responsible for Identification of the Amidotransferase

The reduction step in diaminopimelic acid biosynthesis.

In order to facilitate the elucidation of the remaining undetermined steps in diaminopimelic acid and lysine biosynthesis, mutants blocked in the pathway between aspartic semialdehyde and a-N-succinyl-e-ket.opimelic acid were sought. A mutant (M-203) with a requirement for diaminopimelate and lysine was obtained. An examination of the enzymatic composition of the extract prepared from the mutan...

The enzymatic synthesis of diaminopimelic acid.

a-e-Diaminopimelic acid was discovered by Work in 1949 (1) as a constituent of Corynebacterium diphtheriae. It was also described as occurring in a peptide fraction obtained from Mycobacterium tuberculosis by Asselineau et al. (2). Since the time of these reports distribution of the amino acid has been systematically investigated by Work (3). She found the compound to be generally present in th...